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OUTPUT · 16:9 · PNGThis diagram explains protein synthesis through two linked stages of gene expression: transcription and translation. During transcription, RNA polymerase uses the DNA template strand to synthesize a complementary mRNA molecule. The mRNA carries genetic information from DNA to a ribosome. During translation, the ribosome reads mRNA codons in sequence, while tRNA molecules bring specific amino acids according to their anticodons. The amino acids are joined by peptide bonds to form a growing polypeptide chain, which may fold into a functional protein.
The diagram should show information flowing from DNA to mRNA and then to a polypeptide: DNA → mRNA → protein. RNA polymerase moves along the template strand and builds mRNA in the 5′ to 3′ direction. The ribosome reads the mRNA from 5′ to 3′, beginning at a start codon and ending at a stop codon. Each codon pairs with a complementary tRNA anticodon, ensuring that amino acids are added in the correct order. A codon table must be read using the mRNA codon, not the DNA template or tRNA anticodon.
Use the diagram after introducing DNA structure and before practising genetic-code problems. Ask students to trace the information flow, identify the template strand, mark the 5′ and 3′ ends, and predict the mRNA sequence from a DNA sequence. Then ask which tRNA anticodon and amino acid correspond to each mRNA codon. The figure supports examination points involving complementary base pairing, transcription versus translation, directionality, start and stop codons, and the relationship between nucleotide sequence and amino-acid sequence.
RNA polymerase uses the DNA template strand as a guide and synthesizes a complementary RNA strand. The coding strand has the same base sequence as the mRNA, except that DNA uses thymine while RNA uses uracil.
A codon is a three-base sequence on mRNA that specifies an amino acid or a stop signal. The complementary anticodon on tRNA allows the correct amino acid to be delivered to the ribosome.
The ribosome moves along mRNA from 5′ to 3′, reading codons in order. The polypeptide grows from its N-terminus toward its C-terminus until a stop codon is reached.