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OUTPUT · 16:9 · PNGA genetic pedigree chart shows how a trait or genetic disorder is transmitted through a family. In this three-generation chart, squares represent males and circles represent females. Unshaded symbols indicate normal individuals, fully shaded symbols indicate affected individuals, and half-shaded symbols indicate carriers. For an autosomal recessive disorder, affected individuals have two recessive alleles, while carriers are phenotypically normal but possess one recessive allele. Generation I, Generation II, and Generation III identify successive generations and allow inheritance patterns to be traced over time. The Legend defines every symbol used in the chart.
Individuals in the same generation are arranged on one horizontal level. A horizontal line between a male and a female indicates a mating pair, while a vertical descent line leads to their children, who are joined by a sibship line. In autosomal recessive inheritance, two normal carriers may produce an affected child because each parent can pass on the recessive allele. For a carrier-by-carrier cross, each child has a 25% probability of being affected, a 50% probability of being a carrier, and a 25% probability of inheriting no recessive allele. Males and females are affected with approximately equal frequency.
Use the chart after introducing dominant and recessive alleles and before solving pedigree-analysis problems. Ask students to identify sex, phenotype, generation, and family relationships first, then assign possible genotypes such as AA, Aa, and aa. Questions can progress from “Which individuals are affected?” to “Which normal individuals must be carriers?” and “What is the probability that the next child will be affected?” This activity directly supports assessment of autosomal inheritance, genotype inference, probability calculations, and the distinction between phenotype and genotype.
In autosomal recessive inheritance, both normal parents may be heterozygous carriers. If the child inherits the recessive allele from each parent, the child has genotype aa and expresses the disorder.
In this chart, half-shading represents a known carrier, but symbol conventions should always be checked in the Legend. A suspected carrier may instead be marked with a dot or another notation in some pedigrees.
The trait may skip generations, and affected children can be born to two normal parents. Males and females are usually affected at similar frequencies because the gene is located on an autosome.